Qualification Type: 1 yr MSc + 3 yr PhD

Start Date: September 2015

Location: University of Warwick (MSc Year) University of Oxford (PhD)

Engineering Functionalised Nanodiamond for In-Vivo Imaging of Neural Processes

One of the key scientific challenges of the 21st century is to better understand the principles that allow a collection of neural cells to work as a fully functioning brain. The complexity of neurobiology, along with the range of possible parameters to be investigated means that new techniques are embraced and quickly incorporated. In the Centre for Neural Circuits and Behaviour, a research unit with in the Department of Physiology, Anatomy and Genetics, we use optical and electrical techniques to better understand the brains of fruit flies. 

Nanodiamond (ND) offers many advantages as a fluorescent label for biological applications. Its biocompatibility, potential sensitivity to electric and magnetic fields, lack of photobleaching and the possibility of targetting specific neural structures are all of particular interest to researchers in neurophysiology. This PhD project aims to develop a comprehensive suite of tools and techniques that will enable superresolution imaging of NDs in brain tissue. By utilising Stimulated Emission Depletion (STED) microscopy, in conjunction with adaptive optics, it will be possible to image ND tens of microns into tissue with a resolution of 50nm. Achieving this resolution will allow imaging of synapic sites, the anatomical features where signals are passed from one neuron to another, thereby allowing a better understanding of the propagation of information through the brain. It follows that a key goal for the student will be to obtain a better understanding of how best to functionalise and bind ND to sites of interest, such as synapses, within a brain. 

In order to allow both superresolution imaging, and the later extension to electric and magnetic field sensing, it will also be important to understand how nitrogen-vacancy (NV) centres in the ND are affected by the surface chemistry required for functionalisation, as this may influence the optical activity of the NV centres. 

Due to the requirement to develop the software and hardware required for the successful completion of the PhD, candidates should possess a strong background in physics or engineering alongside any relevant biological and biochemical expertise.

How to apply:

Please e-mail DST.admin<στο>warwick.ac.uk providing a full CV. We will respond as quickly as possible and guide you through the full application process.

Applications should be made early, but will be considered until available places are filled.